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Moisture content determination is one of the important parameters for quality evaluation of crude drug and crude extract. The presence of excess moisture favors the growth of the microorganism and deteriorates the crude drug. So moisture content should be within a specified limit which is acceptable. To perform loss on drying experiments we have to weigh the crucible in an empty state and then measure 0.50 gm of the crude drug than allow it to dry in 100-105 degree for about 15 mint. than place it in a desiccator to cool down and to prevent further moisture absorption and then again place it in the oven till constant weight is received. now finally calculate the moisture content by using the given formula in the video. Procedure: (Drying in Oven): Loss on drying is loss of weight expressed as % w/w resulting from the water and volatile matter of any kind that can be driven off under specified condition.This test is carried out on a well-mixed sample of the substance. If the substance is in the form of large crystals, reduce the size by rapid crushing to a powder. 1. Weigh a glass stoppered, shallow weighing bottle (W1) that has been dried under the same conditions to be employed in determination. 2. Transfer to the bottle the quantity of the sample specified in the individual monograph. Cover it and accurately weigh the bottle and the contents (W2). 3. Distribute the sample as evenly as practicable by gentle sidewise shaking to a depth not exceeding 10 mm. 4. Place the loaded bottle in drying chamber as directed in the monograph per and leave it also in the chamber. Dry the sample to constant weigh or for the specified time and at the temperature indicated in the monograph. 5. After drying is completed, open the drying chamber, close the bottle promptly and allow it to cool to room temperature (where applicable) in a desiccator before weighing. Weighing bottle and contents(W3). 6. When “dry to constant weight” is specified in a monograph, drying shall be continued until two consecutive weighings (W4) do not differ by more than 0.50 mg per g of substance taken, the second weighing following an additional hour of drying. Upon opening the chamber, close the bottle promptly, and allow it to come to room temperature in a desiccator before weighing. 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